Customer Support - Labeling Kits

No. The fluorescent dyes (FAM, TAMRA and ROX) provided with the kits are activated as NHS (N-Hydroxy-Succinamide) esters. NHS esters are reactive with amines. Since Tris is an amine, it will react with the activated dye and act as a scavanger during the labeling reaction. This will reduce the efficiency of the labeling reaction. It is recommended that you exchange your protein into a PBS buffer prior to the labeling reaction using a Centri-Spin spin column (available from Princeton Separations) or related method based on gel filtration. The protein can be exchanged back into the Tris buffer following the labeling reaction.

We typically obtain greater than 90 percent recovery of labeled protein from our spin columns.


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