Introduction:

We have developed a spin column miniprep protocol for the purification of BAC DNA. No organic extractions are required. Typical yields between 0.6 and 1.0 µg are observed from an overnight 3-5 mL culture. A discussion of the data and results depicting the quality of BAC DNA purified is presented. Alternative protocols for preparation of BAC DNA from 25-250mL cultures are also presented. Observations of some factors that affect BAC yield will be discussed.