Can Pro-Spin be used for purification of proteins other than antibodies?

Yes. However, the conditions for purification will vary depending upon the properties of the protein. Recovery of certain proteins may be affected due to hydrophobic or ionic interactions. This must be considered when designing the pH, salt concentration and ionic strength of the hydration buffer. Non-ionic detergents may be necessary for many membrane proteins. Proteins can also be degraded by proteases. Protease degradation can reduce recovery from the column. Appropriate protease inhibitors must be added to the hydration buffer to improve recovery of intact proteins.

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