PSIΨClone PCR ST Single Tube Kit is designed for DNA purification of amplification products from PCR reactions in a single tube format.

This PSIΨClone PCR ST Kit provides a rapid method for processing PCR reaction products in a convenient single tube format. The spin, wash, sample collection tubes and included reagents allow for quick removal of primers, salts, nucleotides and proteins.

DNA fragments (ranging from 100 bp to 10 kb) from PCR reactions bind to the membrane in the spin tube supplied with the kit. Subsequent wash steps remove residual primers, nucleotides,enzymes, and salts. Following wash steps, DNA is eluted in a low salt buffer in high yield and is suitable for further molecular operations without additional processing.

Residual primers, nucleotides, enzymes and salts in PCR amplification reaction products may interfere with molecular operations (e.g. cloning, sequencing etc.). The PSIΨClone PCR ST kit efficiently removes these residual reactants using a convenient single tube format. The purified amplicons are ready for direct sequencing or subcloning.
 

Kit Components and Preparation:

• PCR Spin Tubes: The tubes are supplied ready to use.
• Binding Buffer: This solution is subject to crystallization at temperatures below 22°C. If crystals form, warm the buffer to 50°C to dissolve.
• Wash Buffer: Provided as a concentrate, the Wash Buffer requires dilution with ethanol to a final ethanol concentration of 75-80%. See specific instructions in Protocol sections.
• Elution Buffer: Use as provided.
• Wash Tubes: Provided for use with binding and wash steps.
• Sample Collection Tubes: Provided for use with final elution step.

The PSIΨClone PCR ST DNA purification kit provides sufficient materials to perform 75 separate DNA isolations from standard PCR reactions.

Notes:

1. Follow this protocol for amplicons in the range of 150 bp to 10 kb.
2. For fragments smaller than 150 bp and larger than 70 bp, revise step 1 of the protocol. Use 5 volumes of PCR binding buffer to 1 volume of PCR reaction product. Additional binding buffer will be required and is available as Catalog Number PC-503 (60 mL bottle of binding buffer).

Additional materials required but not supplied:

• Either 95% ethanol or absolute ethanol.
• Suitable centrifuge

Price and order information:

75 Spin Columns with Tubes & Reagents - Product No: PC-511
300 Spin Columns with Tubes & Reagents - Product No: PC-512