PSIΨClone PCR ST Single Tube Kit is designed for DNA purification of amplification products from PCR reactions in a single tube format.
This PSIΨClone PCR ST Kit provides a rapid method for processing PCR reaction products in a convenient single tube format. The spin, wash, sample collection tubes and included reagents allow for quick removal of primers, salts, nucleotides and proteins.
DNA fragments (ranging from 100 bp to 10 kb) from PCR reactions bind to the membrane in the spin tube supplied with the kit. Subsequent wash steps remove residual primers, nucleotides,enzymes, and salts. Following wash steps, DNA is eluted in a low salt buffer in high yield and is suitable for further molecular operations without additional processing.
Residual primers, nucleotides, enzymes and salts in PCR amplification reaction products may interfere with molecular operations (e.g. cloning, sequencing etc.). The PSIΨClone PCR ST kit efficiently removes these residual reactants using a convenient single tube format. The purified amplicons are ready for direct sequencing or subcloning.
The PSIΨClone PCR ST DNA purification kit provides sufficient materials to perform 75 separate DNA isolations from standard PCR reactions.
Notes:
75 Spin Columns with Tubes & Reagents - Product No: PC-511
300 Spin Columns with Tubes & Reagents - Product No: PC-512
Attachment | Size |
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Procedure Guide for PSIΨClone PCR ST | 46.86 KB |