Centri-Sep spin columns are the most effective and reliable column for removal of dye terminators prior to sequencing.
Centri-Sep columns are used for the fast and efficient purification of large molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.)
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Sep unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
The Centri-Sep gel will provide excellent recovery of DNA fragments with sizes greater than 16 base pairs or 25-mer, while removing > 98 % of salts, NTP's and other unwanted low- molecular- weight impurities.
The column gel is hydrated with either reagent-grade water or a suitable buffer or your choice, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through removal of low- molecular- weight components, but also exchanged into the buffer of your choice.
100 Column Kit - Product No: CS-901 Volume Discount Pricing is automatically applied in cart.
Volume Discount Pricing | |
1 - 5 boxes | $316.42 each |
6 - 9 boxes | $300.60 each |
10 - 19 boxes | $292.69 each |
20 - 49 boxes | $284.78 each |
50 + boxes | $268.96 each |
32 Column Kit - Product No: CS-900
Attachment | Size |
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Centri-Sep Procedure.pdf | 151.81 KB |