Immobilized Chymotrypsin (Bovine)

Immobilized Chymotrypsin (Bovine)

Chymotrypsin is a serine endoproteinase which predominantly cleaves peptide bonds on the carboxy side of Tyrosine, Phenylalanine and Tryptophan.

Characteristics:

Chymotrypsin is a serine endoproteinase which predominantly cleaves peptide bonds on the carboxy side of Tyrosine, Phenylalanine and Tryptophan. In addition Chymotrypsin also catalyses hydrolysis at the carboxy side of Leucine, Methionine, Alanine, Aspartic and Glutamic acids, although at a much lower rate. It is therefore recommended to always use the shortest digestion time possible.

Chemical Modification:

Princeton Separations Sequencing Grade Immobilized Bovine Chymotrypsin, TLCK treated, is immobilized on Agarose beads by covalent chemical bonds. The resulting gel is suspended in deionized water at 30% - 50% solids.

Application:

The Sequencing Grade, Immobilized Bovine Chymotrypsin is supplied as a Gel in aqueous suspension and can be used directly from the container without any prior preparations such as washing or reconstitution. The composition of the Immobilized Chymotrypsin Gel is adjusted so that 1 µL of gel suspension is equivalent to 0.5 µg of native Chymotrypsin. A reaction buffer, 1M Triethylamine acetate, pH 8.0 is provided. This buffer is volatile and can be removed easily by Speed-Vac centrifuge.

For accelerated digestion (30 - 60 minutes), a ratio of 1:10 enzyme to protein substrate is recommended. For routine digestion (4 hours - overnight) the recommended ratio is 1:25.

Quality Control:

Two assays are used for Quality Control: an amidase assay (using Benzoyl-Tyrosine-para-nitroanaline as substrate) and a protein digestion assay using casein as substrate. The activity against casein is routinely compared with unmodified Chymotrypsin. Furthermore, the enzyme protein digestion activity is also compared to Trypsin activity and a Trypsin Activity Equivalence is calculated. To check for enzyme specificity an array of synthetic peptides is used.

Price and ordering information:

Product must be shipped overnight

 

Price: $0.00
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Technical Notes for Immobilized Chymotrypsin (Bovine)46.86 KB
Procedure Guide for Immobilized Chymotrypsin (Bovine)46.86 KB

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