Centri-Spin 40 spin columns are used for:
Centri-Spin 40 spin columns are used for the fast and efficient purification of larger molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.)
Centri-Spin 40 gel will retain up to 80 % of short oligonucleotides (< 25-mer) while providing excellent recovery of up to 70 % of large DNA fragments with sizes greater than 135 base pairs. Proteins, peptides, and protein conjugates > 100 kD can also be efficiently separated from unwanted lower molecular-weight impurities.
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. 1 Each Centri-Spin 40 unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
The column gel is hydrated with either reagent-grade water or a suitable buffer, and spun in a microcentrifuge or swinging-bucket Centrifuge to remove the intersititial fluid. After your sample is applied, the column is spun again to remove the low-molecular-weight components, leaving you not only with a purified sample, but one exchanged into the buffer of your choice.
50 Column Kit - Product No: CS-401 Volume discount pricing is automatically applied in cart.
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CentriSpin40 Product Instructions.pdf | 553.22 KB |