CENTRI-SPIN 20

Centri-Spin 20 spin columns are used for:

  • electroporation nick translation binding applications
  • FISH transfection probe labeling
  • gene products signal transduction differential display
  • antibody labeling transformation studies
  • protein labeling receptor analysis
  • solid phase array desalting.

Centri-Spin 20 spin columns are used for the fast and efficient purification of larger molecules (proteins, nucleic acids, complex carbohydrates, etc.), from small molecules (nucleotides, buffer salts, etc.)

The Centri-Spin 20 gel will provide excellent recovery of DNA fragments with sizes greater than 20 base pairs or 20-mer, while removing > 98 % of salts, NTP's and other unwanted low-molecular-weight impurities. Proteins, peptides, and protein conjugates > 25 kD can also be efficiently separated / desalted from unwanted low-molecular-weight impurities.

The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. 1 Each Centri-Spin 20 unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.

The column gel is hydrated with either reagent-grade water or a suitable buffer, and spun in a microcentrifuge or swinging-bucket Centrifuge to remove the intersititial fluid. After your sample is applied, the column is spun again to remove the low-molecular-weight components, leaving you not only with a purified sample, but one exchanged into the buffer of your choice.

Centri-Spin 20 columns can also be used for the following applications:

  • Removal of free and labeled dNTP's from DNA/RNA as in:
  1. nick translation
  2. end-labeling reactions
  3. PCR reactions
  • Improving Electroporation Transformation Efficiencies
  • Removal of hexamers and octamers from primer-walking and random primer labeling
  • Desalting, removal of traces of phenol or exchange of buffer salts, as in multiple restriction digestions
  • Desalting / purification / buffer exchange of peptides or proteins > 25 kD.

These columns are far superior – in their ease of use, speed, and non-toxicity – to such common techniques as phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration.

Advantages of Centri-Spin 20:

  • Processed faster than ultrafiltration columns
  • Rapid and efficient separations
  • Buffer is not preselected
  • Convenient 20–50 µL sample size
  • Dry gel format
  • Room Temperature Storage
  • Microcentrifuge format

Benefits of Centri-Spin 20:

  • Reliable and consistent
  • Time-saving and convenient
  • Enhanced stability
  • Extended shelf-life
  • No special equipment required
  • Extremely versatile

Price and Ordering information:

50 Column Kit - Product No: CS-201 Volume discount pricing is automatically applied in cart.

Volume Discount Pricing
1 - 5 boxes $166.55 each
6 - 9 boxes $158.22 each
10 -19 boxes $154.06 each
20 - 49 boxes $149.90 each
50 - 199 boxes $141.57 each
 

 

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CentriSpin20 Product Instructions.pdf568.87 KB

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