Introduction:

CENTRI-SPIN spin columns provide a convenient and rapid method to remove excess label from nick translation or PCR labeling reactions (see Application Note A-1). For example, CENTRI-SPIN-20 will remove up to 99% of free label from a nick translation reaction in a simple 2 minute centrifugation. CENTRI-SPIN columns are not routinely tested for the presence of RNase and the majority of applications have been developed for proteins and DNA. However, the spin columns may also be used for RNA labeling reactions if precautions are taken to reduce the possibility of RNase contamination. Some of these precautions are discussed below.